Coding

Part:BBa_K1179028

Designed by: Lauren St. Hilaire   Group: iGEM13_MIT   (2013-09-15)

Cas9-VC155 (D10A and H840 mutant)

Coding sequence of Cas9-VC155 fusion protein. The VC155 split venus fluorescent protein is being fused to the Cas9 in order to be able to target multiple sequences of DNA.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3867
    Illegal EcoRI site found at 4478
    Illegal PstI site found at 288
    Illegal PstI site found at 2256
    Illegal PstI site found at 2490
    Illegal PstI site found at 3702
    Illegal PstI site found at 4006
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3867
    Illegal EcoRI site found at 4478
    Illegal PstI site found at 288
    Illegal PstI site found at 2256
    Illegal PstI site found at 2490
    Illegal PstI site found at 3702
    Illegal PstI site found at 4006
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3867
    Illegal EcoRI site found at 4478
    Illegal BglII site found at 295
    Illegal BglII site found at 1232
    Illegal BamHI site found at 1
    Illegal BamHI site found at 2050
    Illegal XhoI site found at 3588
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3867
    Illegal EcoRI site found at 4478
    Illegal PstI site found at 288
    Illegal PstI site found at 2256
    Illegal PstI site found at 2490
    Illegal PstI site found at 3702
    Illegal PstI site found at 4006
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3867
    Illegal EcoRI site found at 4478
    Illegal PstI site found at 288
    Illegal PstI site found at 2256
    Illegal PstI site found at 2490
    Illegal PstI site found at 3702
    Illegal PstI site found at 4006
    Illegal NgoMIV site found at 2784
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None